Isolation and identification of microoragnisms in cassava mill effluent in the soil | Blazingprojects Postgraduate Thesis
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Isolation and identification of microoragnisms in cassava mill effluent in the soil

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Microorganisms
  • 2.2Characteristics of Cassava Mill Effluent
  • 2.3Environmental Impact of Cassava Mill Effluent
  • 2.4Microbial Diversity in Soil
  • 2.5Interaction Between Microorganisms and Effluent
  • 2.6Previous Studies on Microorganisms in Cassava Mill Effluent
  • 2.7Methods for Isolation and Identification of Microorganisms
  • 2.8Role of Microorganisms in Soil Health
  • 2.9Adaptation of Microorganisms to Effluent
  • 2.10Future Research Directions on Microorganisms in Effluent

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Methodology Overview
  • 3.2Sampling and Collection of Cassava Mill Effluent and Soil Samples
  • 3.3Isolation Techniques for Microorganisms
  • 3.4Identification Methods for Microorganisms
  • 3.5Data Analysis Procedures
  • 3.6Quality Control Measures
  • 3.7Ethical Considerations in Research
  • 3.8Statistical Analysis Methods

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Analysis of Microorganisms Isolated from Cassava Mill Effluent
  • 4.2Identification of Dominant Microbial Species
  • 4.3Diversity and Abundance of Microorganisms in Soil
  • 4.4Correlation Between Effluent Characteristics and Microbial Composition
  • 4.5Microbial Interactions and Ecosystem Functioning
  • 4.6Comparison with Previous Studies
  • 4.7Implications for Soil Health and Environmental Management
  • 4.8Recommendations for Future Research

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Conclusion and Summary of Findings
  • 5.2Key Insights from the Research
  • 5.3Contributions to Existing Knowledge
  • 5.4Practical Implications and Applications
  • 5.5Suggestions for Further Studies

Thesis Abstract

The Isolation and identification of microorganisms in cassava mill effluent in the soil in Orji, Imo State, Nigeria were carried out, soil samples were collected from site polluted with cassava mill effluent and also from adjacent site not polluted with cassava effluent to serve as control. The overall population of microorganisms in cassava mill effluent was determined. Isolation and characterization of succession of microorganism in cassava mill effluent was studied microbial count, Bacterial counts and fungal counts were also determined using a pour plate method. The microbial count ranges from 2.2 X 105 – 2.0 X 105 for THBC, 1.1 X 105 – 1.2 X 105 for TCC and the TFC ranges from 1.2 X 105-1.5 X 105. The bacteria isolated were Klebsiella spp., Escherichia coli , Pseudomonas spp., Staphylococcus aureus, and Streptococcus spp. while the fugal isolates include Penicillium spp., Aspergillus spp., mucor spp., and yeast cells Candida spp. the cassava mill effluent should be treated before discharge into the environment to prevent pollution.



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