Isolation and characterization of antibiotic producing actinomycetes in a rhizosphere environment | Blazingprojects Postgraduate Thesis
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Isolation and characterization of antibiotic producing actinomycetes in a rhizosphere environment

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Actinomycetes
  • 2.2Antibiotic Production by Actinomycetes
  • 2.3Rhizosphere Environment
  • 2.4Interaction of Actinomycetes in the Rhizosphere
  • 2.5Isolation Techniques for Actinomycetes
  • 2.6Characterization Methods for Actinomycetes
  • 2.7Importance of Studying Antibiotic Producing Actinomycetes
  • 2.8Impact of Antibiotic Resistance on Actinomycetes
  • 2.9Role of Actinomycetes in Soil Health
  • 2.10Future Prospects in Actinomycetes Research

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design
  • 3.2Sampling Methods
  • 3.3Data Collection Techniques
  • 3.4Experimental Setup
  • 3.5Data Analysis Procedures
  • 3.6Ethical Considerations
  • 3.7Validity and Reliability
  • 3.8Research Limitations

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Overview of Research Findings
  • 4.2Isolation of Antibiotic Producing Actinomycetes
  • 4.3Characterization of Isolated Actinomycetes
  • 4.4Antibiotic Production Assays
  • 4.5Identification of Antibiotic Compounds
  • 4.6Interaction Studies in the Rhizosphere
  • 4.7Comparative Analysis of Antibiotic Activity
  • 4.8Implications of Findings

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion
  • 5.3Recommendations for Future Research
  • 5.4Practical Implications
  • 5.5Contributions to the Field

Thesis Abstract

Two hundred and forty isolates were isolated from the cow dung, compost, soils where the hides and skin of animals were burnt. Three isolates designated A1, A7, and A43 displayed clear zones of inhibition characteristic of Actinomycetes. The isolates were further characterized by examining their colony morphology, cover slip culture techniques, carbon utilization potentials, gram staining reaction and amylase activity. The α-amylase activity was determined on solid medium supplemented with starch. The detection α-amylase activity of the isolates was based on the formation of clear zones around the colonies when flooded with iodine. The decolourization abilities of the isolates were also determined. The isolates showed good absorption characteristics and degrading capacity on the dye Congo red and brilliant green. It showed that Actinomycetes are potential decolorizer of dye stuffs and are good biosorbent.

Thesis Overview

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