Investigating the role of specific microRNAs in regulating gene expression in cancer cells.

Thesis Abstract

Abstract
Cancer is a complex disease characterized by uncontrolled growth and proliferation of cells, often resulting from dysregulation of gene expression. MicroRNAs (miRNAs) are small non-coding RNAs that play crucial roles in post-transcriptional gene regulation and have been implicated in various biological processes, including cancer development and progression. This thesis aims to investigate the role of specific miRNAs in regulating gene expression in cancer cells, with a focus on understanding the mechanisms underlying their dysregulation and potential therapeutic implications. Chapter 1 provides an introduction to the study, outlining the background of the research, problem statement, objectives, limitations, scope, significance, structure of the thesis, and definition of key terms. The literature review in Chapter 2 examines current knowledge on miRNAs, cancer biology, and gene regulation mechanisms, highlighting key findings and gaps in the field. Chapter 3 details the research methodology, including sample collection, cell culture techniques, miRNA profiling, gene expression analysis, and bioinformatics tools utilized for data interpretation. The results obtained in Chapter 4 present a comprehensive analysis of the role of specific miRNAs in cancer cells, identifying dysregulated miRNAs and their target genes associated with cancer progression. The discussion delves into the implications of these findings, potential therapeutic strategies targeting miRNAs, and future research directions. In conclusion, Chapter 5 summarizes the key findings of the study and provides insights into the potential of miRNAs as diagnostic markers and therapeutic targets in cancer. This research contributes to advancing our understanding of the intricate regulatory networks governing gene expression in cancer cells and paves the way for the development of novel miRNA-based therapies for cancer treatment.