Investigating the role of specific microRNAs in regulating gene expression in cancer cells.

Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation. Dysregulation of miRNAs has been implicated in various diseases, including cancer. In this study, we aimed to investigate the role of specific miRNAs in regulating gene expression in cancer cells. The specific objectives were to identify candidate miRNAs involved in cancer progression, elucidate their regulatory mechanisms, and assess their potential as therapeutic targets. Chapter 1 provides an introduction to the study, including background information on miRNAs, the problem statement, research objectives, limitations, scope, significance, structure of the thesis, and definitions of key terms. Chapter 2 presents a comprehensive literature review on miRNA dysregulation in cancer, highlighting key studies and findings related to the role of miRNAs in cancer progression. Chapter 3 details the research methodology employed in this study, including the selection of cancer cell lines, miRNA profiling techniques, gene expression analysis methods, and statistical analyses. The chapter also describes the experimental design and procedures used to investigate the regulatory role of specific miRNAs in cancer cells. Chapter 4 presents a detailed discussion of the findings, including the identification of candidate miRNAs, their target genes, and the regulatory mechanisms involved in gene expression modulation. The chapter also discusses the implications of these findings for cancer biology and potential therapeutic applications. Chapter 5 provides a conclusion and summary of the project thesis, highlighting the key findings, implications, and future research directions. Overall, this study contributes to our understanding of the regulatory role of specific miRNAs in cancer cells and provides insights into their potential as therapeutic targets for cancer treatment.