Investigating the role of microRNAs in regulating gene expression in cancer cells.

Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in post-transcriptional gene regulation. Dysregulation of miRNAs has been implicated in various diseases, including cancer. In this study, we aimed to investigate the role of miRNAs in regulating gene expression in cancer cells, with a focus on understanding how these small molecules contribute to the development and progression of cancer. The research methodology involved the identification and profiling of miRNAs in cancer cells through high-throughput sequencing techniques. Subsequently, bioinformatics analysis was performed to predict potential target genes of the identified miRNAs and assess their functional roles in cancer pathways. Experimental validation of the miRNA-target interactions was carried out using cell culture models and molecular biology techniques. The findings revealed a complex network of interactions between miRNAs and target genes, highlighting the diverse regulatory mechanisms employed by miRNAs in cancer cells. Several key miRNAs were identified as potential regulators of critical oncogenic pathways, providing valuable insights into the molecular mechanisms underlying cancer development. Furthermore, the study demonstrated the potential of miRNAs as diagnostic and therapeutic targets in cancer treatment. The significance of this research lies in its contribution to the understanding of miRNA-mediated gene regulation in cancer cells, paving the way for the development of novel therapeutic strategies targeting dysregulated miRNAs. The results of this study have implications for personalized cancer therapy and precision medicine, offering new opportunities for improving patient outcomes. In conclusion, this thesis provides a comprehensive analysis of the role of miRNAs in regulating gene expression in cancer cells, shedding light on the intricate molecular mechanisms involved in cancer pathogenesis. The findings contribute to the growing body of knowledge on miRNA biology and its implications for cancer research and therapy.