Exploring the role of microRNAs in regulating gene expression in cancer cells: Implications for targeted therapy

Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that play key roles in post-transcriptional regulation of gene expression. In cancer, dysregulation of miRNAs has been implicated in tumorigenesis, metastasis, and drug resistance. This thesis explores the role of miRNAs in regulating gene expression in cancer cells and the potential implications for targeted therapy. Chapter 1 provides an introduction to the study, presenting the background, problem statement, objectives, limitations, scope, significance, and structure of the thesis. The definitions of key terms related to miRNAs, gene expression, cancer cells, and targeted therapy are also provided. Chapter 2 comprises a comprehensive literature review covering ten key aspects related to miRNAs in cancer, including their biogenesis, mechanisms of action, dysregulation in cancer, impact on gene expression networks, and potential therapeutic applications. Chapter 3 details the research methodology, including the study design, cell culture techniques, miRNA profiling methods, gene expression analysis, and bioinformatics tools utilized for data interpretation. The chapter also describes the experimental procedures and statistical analyses employed. Chapter 4 presents a detailed discussion of the findings, highlighting the specific miRNAs identified as key regulators of gene expression in cancer cells. The implications of these findings for targeted therapy strategies, including the development of miRNA-based therapeutics and personalized treatment approaches, are discussed in depth. Chapter 5 concludes the thesis with a summary of the key findings and their significance in the context of cancer biology and therapy. The potential challenges and future directions for research in this field are also addressed, emphasizing the importance of further investigation to harness the therapeutic potential of miRNAs in cancer treatment. Overall, this thesis provides a comprehensive analysis of the role of miRNAs in regulating gene expression in cancer cells and their implications for targeted therapy. The findings contribute to the growing body of knowledge on miRNA-mediated mechanisms in cancer and offer new insights into potential treatment strategies for improving patient outcomes in oncology.