Exploring the role of microRNAs in regulating gene expression in cancer cells

Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that play crucial roles in gene regulation and have been implicated in various diseases, including cancer. This thesis investigates the role of miRNAs in regulating gene expression in cancer cells, with a focus on understanding their mechanisms and potential as therapeutic targets. The study utilized a combination of bioinformatics analysis, cell culture experiments, and molecular biology techniques to elucidate the impact of miRNAs on gene expression in cancer cells. Chapter 1 provides an introduction to the topic, discussing the background of miRNAs, the problem statement, objectives, limitations, scope, significance of the study, and the structure of the thesis. Chapter 2 presents a comprehensive literature review on miRNAs in cancer, highlighting key studies and findings related to their regulatory functions. Chapter 3 outlines the research methodology, including experimental design, cell culture techniques, RNA isolation, and bioinformatics analysis. The results of the study, discussed in Chapter 4, reveal specific miRNAs that are differentially expressed in cancer cells compared to normal cells, suggesting their potential roles in cancer progression. The findings also demonstrate the impact of miRNAs on the expression of target genes involved in critical pathways associated with cancer development and progression. The discussion delves into the implications of these results and their relevance to understanding cancer biology and identifying novel therapeutic strategies targeting miRNAs. In conclusion, Chapter 5 summarizes the key findings of the study and discusses their implications for cancer research and therapy. The study contributes to the growing body of knowledge on the role of miRNAs in cancer and provides insights into potential therapeutic targets for cancer treatment. Overall, this research enhances our understanding of the intricate regulatory networks involving miRNAs in cancer cells and opens new avenues for future investigations and therapeutic interventions.