Estimation of total serum level of protein and iron in mice infected with malaria parasite (plasmodium berghei, berghei) and treated with tetracycline
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of Study
- 1.3Problem Statement
- 1.4Objective of Study
- 1.5Limitation of Study
- 1.6Scope of Study
- 1.7Significance of Study
- 1.8Structure of the Research
- 1.9Definition of Terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Protein and Iron Metabolism
- 2.2Malaria Parasite (Plasmodium berghei, berghei)
- 2.3Tetracycline: Mechanism of Action
- 2.4Previous Studies on Protein and Iron Levels in Mice
- 2.5Effects of Malaria Infection on Protein and Iron Levels
- 2.6Impact of Tetracycline Treatment on Protein and Iron Levels
- 2.7Relationship Between Protein and Iron Levels in Infected Mice
- 2.8Factors Affecting Protein and Iron Assay Accuracy
- 2.9Methods for Estimating Total Serum Protein and Iron
- 2.10Role of Protein and Iron in Immune Response
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Design and Rationale
- 3.2Sampling Methods and Population Selection
- 3.3Data Collection Techniques
- 3.4Experimental Procedures and Treatments
- 3.5Measurement of Total Serum Protein Levels
- 3.6Measurement of Total Serum Iron Levels
- 3.7Statistical Analysis Methods
- 3.8Ethical Considerations and Animal Welfare
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Presentation of Research Findings
- 4.2Analysis of Protein and Iron Levels in Infected Mice
- 4.3Comparison of Protein and Iron Levels pre and post-Treatment
- 4.4Correlation Between Protein and Iron Levels
- 4.5Effects of Tetracycline on Protein and Iron Levels
- 4.6Discussion on Variability in Protein and Iron Assays
- 4.7Interpretation of Results in the Context of Immune Response
- 4.8Implications for Future Research
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Conclusion and Summary of Findings
- 5.2Recap of Research Objectives
- 5.3Contributions to the Field of Study
- 5.4Practical Applications and Future Directions
- 5.5Recommendations for Further Research
Thesis Abstract
The serum has been a center of infection of all sorts, in which most diseases associated with the body revolve, causing alterations in the body’s system. Malaria, as a disease condition of the body (especially in mice) results from inflections with the malaria parasite introduced into the blood stream. (malaria parasite introduced is plasmodium berghei, berghei). Parameters related to the blood of which investigation was concerned were those involving serum protein and iron. A total of 20 mice, 15 with malaria infection, and 5 are non-infected mice (mice) also treated with tetracycline were used for the analysis. The following results were obtained in the end of the study and they are as follows; five animals infected, treated with (10mg/kg, underdose) of tetracycline has the total serum proteins and iron of mean and standard deviation for infected as 3.79 ± 0.96 and 2125.72 ± 947.5 respectively but the mean and standard deviation of control group were, (3.83 ± 1.33) and (1184.2 ± 473.6). At (20mg/kg normal dose = 3.95 ± 0.65) and 1547.14 and the control group were 3.83 ± 1.33 and 1184.2 ± 473.6. At (30mg/kg overdose) has the mean and standard deviation of total proteins and iron as 3.78 ± 0.55 and 1168.58 ± 360. 9 respectively. The reduction in iron level as the dose of the drugs increased is as a result of the chelating ability of tetracycline, significant changes also occurred for proteins. The biochemical implications of these results are these discussed.
Thesis Overview
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