Effect of metal ions on arginase from the kidney of local goat (capra aegagrus hircus)
Table Of Contents
- 1.0 Introduction and Literature review1.1 Introduction1.2 Literature review1.
- 2.1History of arginase1.3 Occurrence and distribution of arginase1.
- 3.1Bacteria1.
- 3.2Fungi1.
- 3.3Lichens1.
- 3.4Yeast1.
- 3.5Insects1.4 The substrate: Arginase1.5 Scientific classification of local goat1.6 Aim and ObjectivesCHAPTER TWO2.0 Materials and methods2.1 Materials2.
- 1.1Apparatus used2.2 Methods2.
- 2.1Assay2.
- 3.2Protein determination2.
- 3.3Purification procedureCHAPTER THREE3.0 Results3.1 Purification and Homogeneity3.2 Effect of metal ion on arginase from kidney of a local goatCHAPTER FOUR5.0 Discussion, Conclusion and Recommendation5.1 Discussion5.2 Conclusion5.3 RecommendationsReferences.
Thesis Abstract
This research is aimed at isolating, partially purifying and determination of arginase from the kidney of a local goat. Methods used in the isolation and partial purification of the enzyme include; Ammonium sulphate precipitation, dialysis.etc. Result from this work showed that arginase isolated from a local goat has a specific activity of 0.358 with a yield of 12.12% incubation of the enzyme with different metal ions shows that the enzyme is inhibited by Nacl and Bacl2.
Thesis Overview
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