EFFECT OF METAL IONS ON ARGINASE FROM THE KIDNEY OF LOCAL GOAT (CAPRA AEGAGRUS HIRCUS) | Blazingprojects Postgraduate Thesis
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EFFECT OF METAL IONS ON ARGINASE FROM THE KIDNEY OF LOCAL GOAT (CAPRA AEGAGRUS HIRCUS)

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Metal Ions
  • 2.2Arginase Enzyme Function
  • 2.3Interaction of Metal Ions with Enzymes
  • 2.4Goat Kidney Physiology
  • 2.5Previous Studies on Metal Ions and Enzymes
  • 2.6Effects of Metal Ions on Biological Systems
  • 2.7Regulation of Metal Ions in Organisms
  • 2.8Importance of Metal Ions in Biological Processes
  • 2.9Methods of Detecting Metal Ions in Biological Samples
  • 2.10Inhibition and Activation of Enzymes by Metal Ions

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Methodology Overview
  • 3.2Study Design and Rationale
  • 3.3Sampling Techniques
  • 3.4Data Collection Methods
  • 3.5Data Analysis Procedures
  • 3.6Instrumentation and Materials Used
  • 3.7Ethical Considerations
  • 3.8Statistical Analysis Techniques

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Data Presentation and Analysis
  • 4.2Effects of Different Metal Ions on Arginase Activity
  • 4.3Comparison of Metal Ion Interactions with Arginase
  • 4.4Relationship Between Metal Ion Concentration and Enzyme Inhibition
  • 4.5Factors Influencing Metal Ion Binding to Arginase
  • 4.6Interpretation of Experimental Results
  • 4.7Discussion on the Implications of Metal Ions on Enzyme Function
  • 4.8Comparison with Previous Studies

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion and Recommendations
  • 5.3Contributions to Existing Knowledge
  • 5.4Implications for Future Research
  • 5.5Reflections on the Research Process

Thesis Abstract

This research is aimed at isolating, partially purifying and determination of arginase from the kidney of a local goat. Methods used in the isolation and partial purification of the enzyme include; Ammonium sulphate precipitation, dialysis.etc. Result from this work showed that arginase isolated from a local goat has a specific activity of 0.358 with a yield of 12.12% incubation of the enzyme with different metal ions shows that the enzyme is inhibited by Nacl and Bacl2.



Thesis Overview

<p> </p><p>1.0 &nbsp; &nbsp; Introduction and Literature review</p><p>1.1 &nbsp; &nbsp; Introduction</p><p>1.2 &nbsp; &nbsp; Literature review</p><p>1.2.1 History of arginase</p><p>1.3 &nbsp; &nbsp; Occurrence and distribution of arginase</p><p>1.3.1 Bacteria</p><p>1.3.2 Fungi</p><p>1.3.3 Lichens</p><p>1.3.4 Yeast</p><p>1.3.5 Insects</p><p>1.4 &nbsp; &nbsp; The substrate: Arginase</p><p>1.5 &nbsp; &nbsp; Scientific classification of local goat</p><p>1.6 &nbsp; &nbsp; Aim and Objectives</p> <br><p></p>

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