Effect of different carbon sources on the growth of antimicrobial producing bacillus species in citrillus vugaris. | Blazingprojects Postgraduate Thesis
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Effect of different carbon sources on the growth of antimicrobial producing bacillus species in citrillus vugaris.

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Antimicrobial Producing Bacillus Species
  • 2.2Carbon Sources and Microbial Growth
  • 2.3Citrullus Vulgaris as a Host Plant
  • 2.4Relationship between Carbon Sources and Antimicrobial Production
  • 2.5Previous Studies on Bacillus Species and Carbon Sources
  • 2.6Impact of Different Carbon Sources on Bacillus Growth
  • 2.7Factors Influencing Antimicrobial Production
  • 2.8Importance of Studying Carbon Sources in Microbial Growth
  • 2.9Methods for Evaluating Bacillus Growth
  • 2.10Role of Carbon Sources in Sustainable Agriculture

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design and Methodology Selection
  • 3.2Sampling Techniques and Sample Size Determination
  • 3.3Data Collection Methods
  • 3.4Experimental Setup and Procedures
  • 3.5Data Analysis Techniques
  • 3.6Quality Control Measures
  • 3.7Ethical Considerations in Research
  • 3.8Limitations of the Research Methodology

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Growth Patterns of Antimicrobial Producing Bacillus Species
  • 4.2Effects of Different Carbon Sources on Bacillus Growth
  • 4.3Comparison of Antimicrobial Production in Various Carbon Sources
  • 4.4Influence of Citrullus Vulgaris on Bacillus Growth
  • 4.5Factors Affecting Antimicrobial Production
  • 4.6Statistical Analysis of Data
  • 4.7Discussion on the Findings
  • 4.8Implications of the Results

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion
  • 5.3Recommendations for Future Research
  • 5.4Practical Applications of the Study
  • 5.5Contribution to the Field

Thesis Abstract

Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus subtilis,(33.33), Bacillus pumulis (41.66), Bacillus licheniformis (25) were identified based on their colony biochemical characteristics.The isolates of the highest occurrences is bacillus pumilus. Agar well diffusion method was used to determine the antimicrobial activity against two pathogenic organisms, Escherichia coli, Staphylococcus aureus. Bacillus subtilis and Bacillus pumulis, gave a zone of inhibition Escherichia coli 0.6mm and 0.8mm after 24 hours of incubation also carbon sources of 5% and 10% sucrose, Glucose and soluble starch were used to check their different carbon sources on the isolates using the spectrophotometer.5% had the highest optimum growth of 1.400 for glucose 1.545 for sucrose,2.376 for soluble starch which can be added in maximum quantity to a medium to produce antibiotics.

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