CHARACTERIZATION OF ARGINASE FROM THE LIVER OF MANGO TILAPIA (Sarotherodongalilaeus)
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of Study
- 1.3Problem Statement
- 1.4Objective of Study
- 1.5Limitation of Study
- 1.6Scope of Study
- 1.7Significance of Study
- 1.8Structure of the Research
- 1.9Definition of Terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Literature Review
- 2.2Historical Perspectives
- 2.3Theoretical Framework
- 2.4Conceptual Framework
- 2.5Empirical Studies
- 2.6Current Trends in the Field
- 2.7Critiques of Existing Literature
- 2.8Research Gaps
- 2.9Methodological Approaches
- 2.10Summary of Literature Reviewed
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Methodology Overview
- 3.2Research Design
- 3.3Data Collection Methods
- 3.4Sampling Techniques
- 3.5Data Analysis Procedures
- 3.6Research Instruments
- 3.7Ethical Considerations
- 3.8Limitations of the Methodology
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Overview of Findings
- 4.2Descriptive Statistics
- 4.3Inferential Statistics
- 4.4Comparison of Results
- 4.5Interpretation of Results
- 4.6Discussion of Findings
- 4.7Implications of Findings
- 4.8Recommendations for Future Research
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Conclusion and Summary of Research
Thesis Abstract
Arginase is a detoxifying enzyme that catalyses the hydrolysis of arginine into ornithine and urea, the last step of urea cycle- a process through which the body disposes off harmful ammonia. This research was carried out to determine the characteristics of liver arginase of mango tilapia (Sarotherodongalilaeus) in Opa river, Osun state. The enzyme ‘arginase’ was isolated from the liver of mango tilapia through the process of homogenization and centrifugation which was done at 4000rev/min.The protein concentration was determined using Bradford method and the arginase assay was determined by Kaysen and Strecker method. The kinetic study shows that mango tilapia of liver arginase has a Km value of 0.2M and a Vmax value of 166.7µmol/ml/min. The effect of temperature on arginase activity was tested and the optimum temperature for mango tilapia liver arginase is 50°C at activity of 63.44µmol/ml/min. The effect of pH was also investigated and optimum pH is 8.0 at activity of 165.1µmol/ml/min. Inhibition study was also carried out and it was observed that calcium (51.4±1.13) and zinc (51.5±4.27) strongly inhibit arginase while mercury, magnesium and sodium have little or no inhibitory effect on arginase. Also from the result, it can be deduce that citrate (20.8±4.67) and glutathione (28.6±2.53) and ethylenediamineacetic acid (EDTA) slightly inhibit arginase while urea has les inhibitory effect on arginase. The result for the effect of amino acids on enzyme activity shows that liver arginase of mango tilapia to be in this order arginine > valine> aspartate > cysteine > lysine with residual activity of 107.2%, 86.6%, 73.4%, 62.1% and 58.7% respectively. Mango tilapia liver arginase belongs to the ureotelic class of arginases according to Mora J et al classification (1965). It has a Km value of 0.2M and aVmax value of 166.7µmol/ml/min.
Thesis Overview
<p> </p><p><strong> INTRODUCTION AND LITERATURE REVIEW</strong></p><p>1.o Introduction</p><p>1.2 Classification</p><p>1.3 Distribution and occurrence</p><p>1.4 Literature review</p><p>1.4.1 Arginase</p><p>1.4.2 Arginase isoenzymes</p><p>1.5 Structural properties</p><p>1.5.1 Molecular properties</p><p>1.5.2 Amino acid composition</p><p>1.6 Physicochemical properties</p><p>1.6.1 Kinetic properties</p><p>1.6.2 Effect of p H</p><p>1.6.3 Effect of temperature</p><p>1.6.4 Effect of metal ions</p><p>1.7 Mechanism of action</p><p>1.8 Arginase deficiency</p><p>1.9 Biological and physiological importance of arginase</p><p>1.10 Study sample</p><p>1.10.1 Taxonomy</p><p>1.10.2 Morphology</p><p>1.10.3 Distribution</p><p>1.10.4 Ecology</p><p>1.10.5 Biology</p><p>1.10.6 Feeding habits</p><p>1.10.7 Reproduction and parental care</p><p>1.10.8 Economic importance</p><p>1.11 Liver</p><p>1.12 Objectives</p> <br><p></p>Blazingprojects Mobile App
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