Antioxidant activity, total phenolic and flavonoid contents of whole plant methanol extract and solvent fractions of desmodiumramosissimum g. don
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of Study
- 1.3Problem Statement
- 1.4Objective of Study
- 1.5Limitation of Study
- 1.6Scope of Study
- 1.7Significance of Study
- 1.8Structure of the Research
- 1.9Definition of Terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Antioxidant Activity
- 2.2Importance of Total Phenolic Content
- 2.3Significance of Flavonoid Content
- 2.4Previous Studies on Desmodiumramosissimum
- 2.5Methods for Extracting Plant Methanol
- 2.6Techniques for Extracting Solvent Fractions
- 2.7Comparison of Different Extraction Methods
- 2.8Factors Affecting Extraction Efficiency
- 2.9Role of Solvents in Extracting Phytochemicals
- 2.10Relationship between Extraction Methods and Phytochemical Contents
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Methodology Overview
- 3.2Selection of Desmodiumramosissimum Plant Samples
- 3.3Preparation of Whole Plant Methanol Extract
- 3.4Fractionation of Solvent Fractions
- 3.5Quantification of Antioxidant Activity
- 3.6Analysis of Total Phenolic Content
- 3.7Determination of Flavonoid Contents
- 3.8Statistical Analysis Methods
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Antioxidant Activity Results
- 4.2Total Phenolic Content Findings
- 4.3Flavonoid Content Discoveries
- 4.4Comparison of Methanol Extract and Solvent Fractions
- 4.5Interpretation of Extraction Efficiency
- 4.6Correlation between Extraction Methods and Phytochemical Contents
- 4.7Discussion on Factors Influencing Results
- 4.8Implications of Findings for Desmodiumramosissimum
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Summary of Research Findings
- 5.2Conclusion on Antioxidant Activity Study
- 5.3Recommendations for Future Research
- 5.4Contribution to Existing Knowledge
- 5.5Final Thoughts and Reflections
Thesis Abstract
The present study was designed to estimate the total phenolic and flavonoid contents and to evaluate the in vitro antioxidant activities of both the methanol extract and the various solvent fractions of the whole plant, Desmodiumramosissimum. Qualitative phytochemical screening showed the presence of various bioactive components such as phenolics, flavonoids and tannins. The quantitative analyses of total phenolic content (TPC) and total flavonoid content (TFC) were carried out spectrophotometrically using Folin-Ciocalteu phenol reagent and aluminum chloride method, respectively. Ethyl acetate fraction followed by n-butanol fraction exhibited high total antioxidant activity, DPPH radical scavenging ability as well as ferric ion reducing ability when compared to all other solvent fractions including the methanol extract. The presence of these flavonoid and phenolic compounds might contribute to the antioxidant properties observed in these fractions. In order to support this observation, the relationship between the antioxidant content using various models revealed that TPC exhibited a strong correlation with ferric ion reducing potential (FIRP) and total antioxidant capacity (TAC) with R2 vmalues of 0.995 and 0.980, respectively; but a weak relationship with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical with R2 value of 0.877. However, aqueous fraction showed a strong relationship between DPPH and its concentration compared to other fractions. This showed that various solvent extracts possess unique chemical constituent of plant with various antioxidant capacity. Free radicals were scavenged by the extract and solvent fractions in a concentration-dependent manner within the range of the given concentrations (25-1000 mg/l) in all models. The findings from this study show that methanol extract and the solvent fractions of Desmodiumramosissimum G. Don contained phenolics, flavonoids and tannins which exhibited antioxidant properties.
Thesis Overview
<p> The present study was designed to estimate the total phenolic and flavonoid contents and to evaluate the in vitro antioxidant activities of both the methanol extract and the various solvent fractions of the whole plant, Desmodiumramosissimum. Qualitative phytochemical screening showed the presence of various bioactive components such as phenolics, flavonoids and tannins. The quantitative analyses of total phenolic content (TPC) and total flavonoid content (TFC) were carried out spectrophotometrically using Folin-Ciocalteu phenol reagent and aluminum chloride method, respectively. Ethyl acetate fraction followed by n-butanol fraction exhibited high total antioxidant activity, DPPH radical scavenging ability as well as ferric ion reducing ability when compared to all other solvent fractions including the methanol extract. The presence of these flavonoid and phenolic compounds might contribute to the antioxidant properties observed in these fractions. In order to support this observation, the relationship between the antioxidant content using various models revealed that TPC exhibited a strong correlation with ferric ion reducing potential (FIRP) and total antioxidant capacity (TAC) with R2 vmalues of 0.995 and 0.980, respectively; but a weak relationship with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical with R2 value of 0.877. However, aqueous fraction showed a strong relationship between DPPH and its concentration compared to other fractions. This showed that various solvent extracts possess unique chemical constituent of plant with various antioxidant capacity. Free radicals were scavenged by the extract and solvent fractions in a concentration-dependent manner within the range of the given concentrations (25-1000 mg/l) in all models. The findings from this study show that methanol extract and the solvent fractions of Desmodiumramosissimum G. Don contained phenolics, flavonoids and tannins which exhibited antioxidant properties. <br></p>Blazingprojects Mobile App
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