A SURVEY OF THE ANTIBACTERIAL ACTIVITY OF THYMUS VULGARIS AND MYRISTICA FRAGRANS ON STREPTOCOCUSS PNEUMONIAE AND PSEUDOMONAS AERUGINOSA | Blazingprojects Postgraduate Thesis
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A SURVEY OF THE ANTIBACTERIAL ACTIVITY OF THYMUS VULGARIS AND MYRISTICA FRAGRANS ON STREPTOCOCUSS PNEUMONIAE AND PSEUDOMONAS AERUGINOSA

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Antibacterial Properties
  • 2.2Thymus Vulgaris and Its Antibacterial Activity
  • 2.3Myristica Fragrans and Its Antibacterial Activity
  • 2.4Streptococcus Pneumoniae: Characteristics and Antibiotic Resistance
  • 2.5Pseudomonas Aeruginosa: Characteristics and Antibiotic Resistance
  • 2.6Previous Studies on Thymus Vulgaris and Myristica Fragrans
  • 2.7Comparison of Antibacterial Activity of Thymus Vulgaris and Myristica Fragrans
  • 2.8Mechanisms of Antibacterial Action
  • 2.9Factors Affecting Antibacterial Activity
  • 2.10Future Directions for Research

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Methodology Overview
  • 3.2Research Design
  • 3.3Sampling Techniques and Sample Size
  • 3.4Data Collection Methods
  • 3.5Data Analysis Techniques
  • 3.6Experimental Setup
  • 3.7Variables and Controls
  • 3.8Ethical Considerations

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Data Analysis and Interpretation
  • 4.2Antibacterial Activity of Thymus Vulgaris on Streptococcus Pneumoniae
  • 4.3Antibacterial Activity of Thymus Vulgaris on Pseudomonas Aeruginosa
  • 4.4Antibacterial Activity of Myristica Fragrans on Streptococcus Pneumoniae
  • 4.5Antibacterial Activity of Myristica Fragrans on Pseudomonas Aeruginosa
  • 4.6Comparison of Antibacterial Effects Between Thymus Vulgaris and Myristica Fragrans
  • 4.7Discussion on Mechanisms of Action
  • 4.8Implications of Findings

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion
  • 5.3Recommendations for Future Research
  • 5.4Practical Applications of the Study

Thesis Abstract

The aqueous and ethanolic extracts of the leaves of Thymus vulgaris (thyme) and the seeds of Myristica fragrans (nutmeg) were screened for their antibacterial activity and phytochemical constituents. The standard methods used to detect the phytochemicals present in the extracts revealed the presence of tannins, flavonoids, triterpenoids and phytosterols in all the extracts while steroids and amino acids were absent. The antibacterial evaluation of the spice extracts using agar plate-well diffusion and nutrient broth dilution techniques showed thatthere was no significant difference in the activity of the spice extracts (p > 0.05) against the test organisms (Streptococcus pneumoniaeand Pseudomonas aeruginosa, clinical bacterial isolates). Ampiclox and chloramphenicol were used as control for the test organisms to provide approximate basis for the comparison of the activity of the extracts. The MIC and MBC was the same for all the extracts against S. pneumoniae, 6.25 mg/ml while 25 mg/ml for thyme extracts, and 50 mg/ml for nutmeg extracts against P. aeruginosa, showing the extracts to be bactericidal.



Thesis Overview

<p> </p><p>The spread of drug-resistant pathogens is one of the most serious threats to public health in the 21st century. Although pharmacological industries have produced a number of new antibiotics in the last three decades, resistance to these drugs by <a target="_blank" rel="nofollow" href="https://www.projecttopics.org/the-role-of-plants-in-the-treatment-of-diseases-caused-by-micro-organisms-based-in-the-natural-products.html">micro-organisms</a>&nbsp;still remains. In general, bacteria have the genetic ability to transmit and acquire resistance to drugs, which are utilized as therapeutic agents (Cohen, 1992). Such a fact is a cause for concern, because of the number of patients in hospitals who have suppressed immunity, and due to new bacterial strains that are multi-drug resistant. Consequently, new infections can occur in hospitals resulting in high mortality as observed by Montelli and Levy (1991).</p><p>The use of herbs as first medicines is a universal phenomenon. For a long period of time, plants have been a valuable source of natural products for maintaining human health, especially in the last decade, with more intensive studies for natural therapies. Most drugs of the past were substances with a particular therapeutic action extracted from plants. Thus, medicinal plants may be defined as any plant that can be put to culinary use such as onions, fox glove, garlic and ginger (Wainright, 2001). More and more researchers find that food and their individual constituents perform similar fashion to modern drugs and sometimes better without the dreaded side effects (Serrentino, 1991).</p><p>Plant extracts have great potential as antimicrobial compounds against <a target="_blank" rel="nofollow" href="https://www.projecttopics.org/the-role-of-plants-in-the-treatment-of-diseases-caused-by-micro-organisms-based-in-the-natural-products.html">micro-organisms</a>&nbsp;(Gisleneet al., 2000) and studies have reported that they can be used in the treatment of infectious diseases caused by resistant microbes.Thymus vulgaris (thyme) leaves andMyristicafragrans(nutmeg) seeds are plants parts that are widely used as spices for cooking especially for flavouring meats, soups and stews. They have also been reported to have medicinal uses and possess antimicrobial abilities(Stahl-BiskupandSaez, 2002; Cho et al., 2006; Na</p> <br><p></p>

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