Exploring the Role of MicroRNAs in Regulating Gene Expression in Human Skeletal Muscle Cells

Thesis Abstract

Abstract
This thesis explores the intricate mechanisms by which MicroRNAs (miRNAs) regulate gene expression in human skeletal muscle cells. The study delves into the significance of miRNAs in controlling various cellular processes essential for muscle development, maintenance, and function. The research aims to provide a comprehensive understanding of how miRNAs influence gene expression in skeletal muscle cells and the implications of dysregulated miRNA activity in muscle-related disorders. Chapter 1 provides an introduction to the study, outlining the background, problem statement, objectives, limitations, scope, significance, structure of the thesis, and definitions of key terms related to miRNAs and gene expression in skeletal muscle cells. The chapter sets the stage for the subsequent discussions on the role of miRNAs in muscle biology. Chapter 2 presents a detailed literature review encompassing ten key areas related to miRNA function in skeletal muscle cells. The review synthesizes existing knowledge on miRNA-mediated gene regulation, muscle development, regeneration, metabolism, and disease pathogenesis. It highlights the critical findings and gaps in current research, guiding the subsequent investigations in this thesis. Chapter 3 outlines the research methodology employed to investigate the role of miRNAs in regulating gene expression in human skeletal muscle cells. The chapter describes the experimental design, cell culture techniques, miRNA profiling methods, gene expression analysis approaches, and statistical tools utilized to elucidate the molecular mechanisms underlying miRNA-mediated gene regulation in muscle cells. Chapter 4 presents a comprehensive discussion of the research findings, addressing the impact of specific miRNAs on gene expression patterns in skeletal muscle cells. The chapter examines the regulatory networks orchestrated by miRNAs and their implications for muscle cell function, differentiation, and adaptation to physiological stimuli. It explores the interplay between miRNAs and target genes in shaping the molecular landscape of skeletal muscle biology. Chapter 5 offers a conclusive summary of the thesis, highlighting the key insights gained from the study on the role of miRNAs in regulating gene expression in human skeletal muscle cells. The chapter emphasizes the significance of miRNA-mediated gene regulation in muscle physiology and pathology, underscoring the potential therapeutic implications of targeting miRNAs for treating muscle-related disorders. In conclusion, this thesis contributes to the growing body of knowledge on the regulatory functions of miRNAs in skeletal muscle cells. By unraveling the complex interplay between miRNAs and gene expression networks, this research sheds light on novel avenues for understanding and manipulating muscle biology for therapeutic interventions in muscle disorders.