Thesis Abstract

Abstract
This thesis focuses on the investigation of the role of MicroRNAs (miRNAs) in the diagnosis and prognosis of cancer within the field of Medical Laboratory Science. MicroRNAs are small non-coding RNAs that play crucial roles in gene regulation and have been implicated in various diseases, including cancer. The study aims to explore the potential of miRNAs as biomarkers for cancer diagnosis and prognosis, with the ultimate goal of improving patient outcomes through early detection and personalized treatment strategies. Chapter 1 provides an introduction to the research topic, highlighting the background of the study, the problem statement, objectives of the study, limitations, scope, significance, and structure of the thesis. In Chapter 2, a comprehensive literature review is conducted to examine the current understanding of miRNAs in cancer, including their dysregulation in different cancer types and their potential as diagnostic and prognostic biomarkers. Ten key areas of focus are identified and analyzed to provide a solid foundation for the research. Chapter 3 outlines the research methodology employed in this study, including the selection of study participants, sample collection and processing, miRNA profiling techniques, data analysis methods, and statistical approaches. Eight key components of the research methodology are discussed to ensure the rigor and validity of the study findings. In Chapter 4, the research findings are presented and discussed in detail, focusing on the identification of specific miRNAs associated with cancer diagnosis and prognosis. The results are interpreted in the context of existing literature and potential implications for clinical practice. Various aspects related to miRNA expression patterns, target genes, and functional roles are explored to provide a comprehensive analysis of the findings. Chapter 5 serves as the conclusion and summary of the thesis, highlighting the key findings, implications for future research, and practical applications in the field of Medical Laboratory Science. The study underscores the importance of miRNAs as promising biomarkers for cancer diagnosis and prognosis and emphasizes the potential for personalized medicine approaches in cancer management. Overall, this thesis contributes to the growing body of knowledge on the role of miRNAs in cancer diagnosis and prognosis, offering valuable insights into the potential applications of miRNA-based biomarkers in clinical practice. By elucidating the molecular mechanisms underlying cancer development and progression, this research aims to advance the field of Medical Laboratory Science and ultimately improve patient care and outcomes in the fight against cancer.

Thesis Overview

The project titled "Investigating the Role of MicroRNAs in Cancer Diagnosis and Prognosis in Medical Laboratory Science" aims to explore the significance of microRNAs in the field of cancer diagnosis and prognosis. MicroRNAs are small non-coding RNA molecules that play crucial roles in regulating gene expression and are increasingly recognized as potential biomarkers for various diseases, including cancer. This research seeks to investigate how specific microRNAs can be utilized in medical laboratory science to improve the accuracy and efficiency of cancer diagnosis and prognosis. The project will begin with a comprehensive literature review to establish the current understanding of microRNAs in cancer research. This review will cover topics such as the role of microRNAs in cancer development, their potential as diagnostic and prognostic markers, and the methods used to analyze microRNA expression levels in clinical samples. By synthesizing existing knowledge in this area, the research aims to identify gaps in the current literature and opportunities for further investigation. The methodology chapter will outline the research design and approach to be used in this study. This will include details on sample collection, RNA extraction and analysis techniques, as well as statistical methods for data interpretation. The research will focus on identifying specific microRNAs that show differential expression patterns in cancer patients compared to healthy individuals, with the goal of developing a panel of microRNA biomarkers for cancer diagnosis and prognosis. The findings chapter will present the results of the study, including the identification of candidate microRNAs with diagnostic and prognostic potential. The discussion will analyze these findings in the context of existing literature and provide insights into the clinical implications of using microRNAs as biomarkers in cancer management. The limitations of the study will also be addressed, along with recommendations for future research in this area. In conclusion, this research project has the potential to contribute significantly to the field of medical laboratory science by elucidating the role of microRNAs in cancer diagnosis and prognosis. By identifying specific microRNA biomarkers and developing robust analytical methods for their detection, this study aims to enhance the accuracy and efficiency of cancer diagnosis and prognosis, ultimately leading to improved patient outcomes.