<p>1. Introduction<br> 1.1 Background<br> 1.2 Significance of the Study<br> 1.3 Research Objectives<br><br>2. Literature Review<br> 2.1 Gut Microbiota in Piglets<br> 2.1.1 Composition and Function<br> 2.1.2 Factors Influencing Gut Microbiota<br> 2.2 Weaning Strategies in Pig Production<br> 2.2.1 Traditional Abrupt Weaning<br> 2.2.2 Two-Step Weaning<br> 2.2.3 Step-Down Weaning<br> 2.2.4 Other Weaning Approaches<br> 2.3 Previous Studies on Weaning and Gut Microbiota in Piglets<br> 2.3.1 Effects of Abrupt Weaning<br> 2.3.2 Effects of Two-Step Weaning<br> 2.3.3 Effects of Step-Down Weaning<br> 2.3.4 Other Findings<br><br>3. Methodology<br> 3.1 Experimental Design<br> 3.2 Sample Collection<br> 3.3 DNA Extraction and Sequencing<br> 3.4 Bioinformatics Analysis<br> 3.5 Statistical Analysis<br><br>4. Results<br> 4.1 Gut Microbiota Composition in Piglets<br> 4.2 Effects of Different Weaning Strategies on Gut Microbiota<br> 4.3 Comparison of Microbial Diversity and Abundance<br> 4.4 Identification of Key Microbial Taxa<br><br>5. Discussion<br> 5.1 Impact of Weaning Strategies on Gut Microbiota<br> 5.2 Potential Mechanisms<br> 5.3 Implications for Piglet Health and Nutrition<br> 5.4 Limitations and Future Directions <br></p>
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