Thesis Abstract

Abstract
This thesis explores the intricate mechanisms by which MicroRNAs (miRNAs) regulate gene expression in human skeletal muscle cells. The study delves into the significance of miRNAs in controlling various cellular processes essential for muscle development, maintenance, and function. The research aims to provide a comprehensive understanding of how miRNAs influence gene expression in skeletal muscle cells and the implications of dysregulated miRNA activity in muscle-related disorders. Chapter 1 provides an introduction to the study, outlining the background, problem statement, objectives, limitations, scope, significance, structure of the thesis, and definitions of key terms related to miRNAs and gene expression in skeletal muscle cells. The chapter sets the stage for the subsequent discussions on the role of miRNAs in muscle biology. Chapter 2 presents a detailed literature review encompassing ten key areas related to miRNA function in skeletal muscle cells. The review synthesizes existing knowledge on miRNA-mediated gene regulation, muscle development, regeneration, metabolism, and disease pathogenesis. It highlights the critical findings and gaps in current research, guiding the subsequent investigations in this thesis. Chapter 3 outlines the research methodology employed to investigate the role of miRNAs in regulating gene expression in human skeletal muscle cells. The chapter describes the experimental design, cell culture techniques, miRNA profiling methods, gene expression analysis approaches, and statistical tools utilized to elucidate the molecular mechanisms underlying miRNA-mediated gene regulation in muscle cells. Chapter 4 presents a comprehensive discussion of the research findings, addressing the impact of specific miRNAs on gene expression patterns in skeletal muscle cells. The chapter examines the regulatory networks orchestrated by miRNAs and their implications for muscle cell function, differentiation, and adaptation to physiological stimuli. It explores the interplay between miRNAs and target genes in shaping the molecular landscape of skeletal muscle biology. Chapter 5 offers a conclusive summary of the thesis, highlighting the key insights gained from the study on the role of miRNAs in regulating gene expression in human skeletal muscle cells. The chapter emphasizes the significance of miRNA-mediated gene regulation in muscle physiology and pathology, underscoring the potential therapeutic implications of targeting miRNAs for treating muscle-related disorders. In conclusion, this thesis contributes to the growing body of knowledge on the regulatory functions of miRNAs in skeletal muscle cells. By unraveling the complex interplay between miRNAs and gene expression networks, this research sheds light on novel avenues for understanding and manipulating muscle biology for therapeutic interventions in muscle disorders.

Thesis Overview

The project titled "Exploring the Role of MicroRNAs in Regulating Gene Expression in Human Skeletal Muscle Cells" aims to delve into the intricate mechanisms underlying gene expression regulation in human skeletal muscle cells. This research seeks to investigate the critical role of microRNAs, small non-coding RNA molecules, in modulating gene expression patterns within skeletal muscle cells. By understanding how microRNAs influence gene expression, the study aims to uncover new insights into the regulatory processes that govern skeletal muscle function and development. The human skeletal muscle system plays a fundamental role in supporting movement and physical activity. Gene expression within skeletal muscle cells is tightly regulated to ensure proper muscle function, adaptation to exercise, and response to various physiological stimuli. MicroRNAs have emerged as key players in post-transcriptional gene regulation, acting by binding to target mRNAs and modulating their stability and translation efficiency. Despite their importance, the specific roles of microRNAs in regulating gene expression in skeletal muscle cells remain incompletely understood. This research project will employ a multidisciplinary approach, integrating molecular biology techniques, bioinformatics analyses, and cell culture experiments to investigate the impact of microRNAs on gene expression in human skeletal muscle cells. By profiling the expression patterns of microRNAs and their target genes, this study aims to identify key regulatory networks involved in controlling skeletal muscle gene expression. Furthermore, the project will explore the functional consequences of manipulating specific microRNAs on skeletal muscle cell behavior, such as differentiation, proliferation, and metabolic activity. By elucidating the regulatory roles of microRNAs in skeletal muscle cells, this research has the potential to uncover novel therapeutic targets for treating muscle-related disorders and enhancing muscle performance. Overall, this project seeks to advance our understanding of how microRNAs contribute to the intricate regulatory networks governing gene expression in human skeletal muscle cells. By shedding light on these molecular mechanisms, this research aims to provide valuable insights that could lead to the development of innovative strategies for improving skeletal muscle health and function."