Project Abstract

This project aims to investigate the antimicrobial properties of natural plant extracts against drug-resistant pathogenic bacteria and fungi. The rise of antimicrobial resistance (AMR) has become a global public health concern, with many commonly used antibiotics becoming increasingly ineffective against a wide range of infectious agents. This issue has prompted the scientific community to explore alternative antimicrobial strategies, with a particular focus on the potential of natural plant-based compounds. Natural products, such as those derived from medicinal plants, have long been recognized for their diverse pharmacological activities, including antimicrobial properties. These plant-based compounds often possess complex chemical structures and multiple mechanisms of action, which can make them less susceptible to the development of resistance compared to synthetic antibiotics. Furthermore, plant extracts may offer a more holistic approach to antimicrobial therapy, as they often contain a variety of bioactive molecules that can target multiple pathways in microbial cells. The primary objective of this project is to identify and characterize the antimicrobial potential of selected plant extracts against a panel of drug-resistant bacterial and fungal strains. This will involve a systematic screening of various plant species, followed by the isolation and purification of the active antimicrobial compounds. The project will also explore the underlying mechanisms of action of the most promising plant-derived antimicrobials, including their mode of inhibition, target specificity, and synergistic effects with conventional antibiotics. The research methodology will include a combination of in vitro and in vivo approaches. Initially, plant samples will be collected, extracted, and screened for their antimicrobial activity against a diverse collection of drug-resistant pathogens, including Methicillin-resistant Staphylococcus aureus (MRSA), Carbapenem-resistant Enterobacteriaceae (CRE), and Candida auris. The most potent extracts will then be subjected to further fractionation and chemical characterization to identify the active antimicrobial compounds. The project will also investigate the potential synergistic interactions between the plant-derived antimicrobials and conventional antibiotics. This approach could lead to the development of novel antimicrobial therapies that can enhance the efficacy of existing drugs and potentially reduce the emergence of resistant strains. In addition to the antimicrobial evaluation, the project will assess the safety and cytotoxicity profiles of the selected plant extracts and purified compounds using appropriate in vitro and in vivo models. This information will be crucial for the potential translation of the research findings into clinical applications. The successful completion of this project is expected to contribute to the growing body of knowledge on the antimicrobial potential of natural plant-based compounds. The identification of effective and safe antimicrobial agents from natural sources could lead to the development of novel therapeutic options for the management of drug-resistant infections, ultimately improving patient outcomes and public health. Furthermore, the insights gained from this research may also inspire the discovery of new antimicrobial lead compounds and the design of more effective and sustainable antimicrobial strategies.

Project Overview