Project Abstract

This project aims to investigate the antibiotic resistance profiles of Escherichia coli (E. coli) strains isolated from clinical settings and the surrounding environment. Escherichia coli is a ubiquitous bacterium that plays a crucial role in both human health and environmental ecosystems. While E. coli is commonly found in the human gut and is generally harmless, certain strains can cause severe gastrointestinal infections and pose a significant public health concern. The emergence and spread of antibiotic-resistant E. coli strains have become a growing global challenge, posing a threat to effective treatment and control of infectious diseases. The importance of this project lies in its potential to shed light on the complex relationship between clinical and environmental E. coli isolates, and their respective antibiotic resistance patterns. By conducting a comparative analysis, the study aims to gain a deeper understanding of the mechanisms and factors contributing to the development and dissemination of antibiotic resistance in this versatile bacterium. The project will involve the collection of E. coli isolates from various clinical sources, such as hospital settings and outpatient clinics, as well as from environmental samples, including water bodies, soil, and agricultural areas. These isolates will be subjected to comprehensive antimicrobial susceptibility testing, using a standard panel of antibiotics commonly used in clinical practice and environmental settings. The data collected from the antimicrobial susceptibility testing will be carefully analyzed to identify and compare the resistance patterns observed in clinical and environmental E. coli isolates. This analysis will include the evaluation of resistance rates, the prevalence of multidrug-resistant strains, and the identification of specific resistance mechanisms, such as the production of enzymes that inactivate antibiotics or the presence of efflux pumps that expel them from the bacterial cells. Furthermore, the project will explore the potential genetic and molecular mechanisms underlying the observed antibiotic resistance patterns. This will involve the use of advanced molecular techniques, such as whole-genome sequencing and bioinformatics analysis, to identify the presence of resistance genes and understand their genetic context and potential for horizontal transfer between different E. coli strains or even to other bacterial species. The findings of this project will have important implications for public health and environmental management. By elucidating the similarities and differences in antibiotic resistance profiles between clinical and environmental E. coli isolates, the study will provide valuable insights into the complex interplay between human health, animal health, and the environment. These insights can inform the development of more effective strategies for the prevention and control of antibiotic-resistant E. coli, including the implementation of appropriate antibiotic stewardship programs, improved wastewater treatment processes, and the implementation of effective biosecurity measures in agricultural settings. Moreover, the project's findings may contribute to the scientific understanding of the ecology and evolution of antibiotic resistance in E. coli, which can have far-reaching implications for the development of novel antimicrobial interventions and the preservation of the efficacy of existing antibiotics.

Project Overview