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Preliminary phytochemical and antimicrobial screening of flower and stalk of mangifera indica (opioro mango)

 

Table Of Contents


Project Abstract

Phytochemical and antimicrobial screening analysis of Mangifera indica indicated that the presence of the following moisture content 12.33% +0.0556%, Alkaloid     7.35% +0.0291%, Tannins 18.22% +0.0070%, Saponic Glycoside 3.82% +0.0264%, Flanonoid 6.11% + 0.0158%, Phenolic glycoside 0.010% + 0.0012%, Terpenoids 5.71% + 0.01%, Antrachionic 10.59% + 0.0158%, pH 3.84% + 0.0529%. Cardiac Glycoside 7.90% + 0.0234%. Extracts of Mangifera indica were investigated for its antifungal and antibacterial activities. The aqueous extract did not inhibit any of the test fungi, the ethanolic extract produced zones of inhibition against Staphylococcus aureus, S. pyogens, Escherichia coli, Bacilus subfilis, while C. albican and a. flavus were not inhibited.

Project Overview

                                           Title page                                                                               Declaration                                                                                             Certification                                                                                     Dedication                                                                                               Acknowledgment                                                                                   Table of content                                                                           Abstract                                                                                          

CHAPTER ONE 1.0   INTRODUCTION                                                                           OBJECTIVE OF THIS RESEARCH                                   1.1   LITERATURE REVIEW                                                       1.2   GENERAL USES AND IMPORTANCE OF MANGIFERA INDICA                                                         1.3   MORPHLOGY OF MANGIFERA INDICA; THE TREE AND ITS FLOWERS                                                                                      CHAPTER TWO 2.0   MATERIALS AND METHODS                                           2.1   MATERIALS                                                                         2.2   EQUIPMENTS AND APPARATUS                                             2.3   CHEMICAL REAGENTS                                                     2.4   PHYTOCHEMICAL SCREENING (QUANTITATIVE       ANALYSIS)                                                                           2.5   DETERMINATION OF SAPONIN                                       2.6   DETERMINATION OF ALKALOID                                               2.7   DETERMINATION OF TANINS                                         2.8   DETERMINATION OF MOISTURE CONTENT               2.9   DETERMINATION OF TOTAL ASH                                   2.10 CARDIAC GLYCOSIDE                                                       2.11 TEST FOR ANTHRACENE GLYCOSIDES                       2.12 TERPENOIDS                                                                       2.13 pH AT 200C (10%)                                                                CHAPTER THREE TABLE 3.1 ORGANOLEPTIC TEST RESULT                         TABLE 3.2 RESULT OF THE PHYTOCHEMICAL ANALYSIS OF FLOWER AND STALK OF Mangifera Indica     TABLE 3.3RESULT OF ANTI-BACTERIAL ACTIVITY OF TWO GRAM POSITIVE BACTERIA IN TWO CRUDE EXTRACTS OF FLOWER AND STALK OF Mangifera indica                                                          TABLE 3.4 RESULT OF ANTI-BACTERIAL ACTIVITY OF TWO GRAM NEGATIVE BACTERIA IN TWO CRUDE EXTRACTS OF FLOWER AND STALK OF Mangifera indica                                                    TABLE 3.5 RESULT OF ANTI-FUNGAL ACTIVITY OF TWO FUNGI IN TWO CRUDE EXTRACTS OF FLOWER AND STALK OF Mangifera indica                                                    TABLE 3.6 RESULT OF MINIMUM INHIBITORY CONCENTRATION (MIC) OF CRUDE EXTRACT OF FLOWER AND STALK OF Mangifera indica                                                    CHAPTER FOUR DISCUSSION                                                             CONCLUSION                                                             RECOMMENDATION                                                 REFERENCES                                                           APPENDIX                                                                          


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