Home / Biology edcuation / 1.0 Introduction 1.1 Medicinal Plants 1.2 Aim of Study CHAPTER TWO 2.0 Literature Review 2.1 Application of medicinal plants 2.1.1 Diocleareflexa Hook. F 2.2 Lipid Profile 2.2.1 Impacts of medicinal plants on lipid profile CHAPTER THREE 3.0 Materials and Methods 3.1 Apparatus and Equipments 3.2 Chemical Reagents 3.3 Collection and extraction of plant materials 3.3.1 Diocleareflexa Hook. F 3.4 Practical history and animal management 3.5 Drug administration 3.6 Sacrificing of rats 3.7 Biochemical analysis 3.7.1 Triglycerides 3.7.1.1 Principle 3.7.1.2 Reagent composition 3.7.1.3 Preparation of reagents 3.7.1.4 Procedure 3.7.2 HDL Cholesterol 3.7.2.1 Principle 3.7.2.2 Preparation of reagents 3.7.2.3 Procedure 3.8 Statistical analysis CHAPTER FOUR 4.0 Results CHAPTER FIVE 5.0 Discussion, Conclusion and Recommendation 5.1 Discussion 5.2 Conclusion 5.3 Recommendations References Appendix LIST OF FIGURES Figure1 – Diocleareflexa Hook. F Figure2 – Bar chart showing the level of cholesterol in adult wistar rats Figure3 – Bar chart showing the level of HDL in adult wistar rats Figure4 – Bar chart showing the level of LDL in adult wistar rats Figure5 – Bar chart showing the level of triglycerides in adult wistar rats LIST OF TABLE Table 1 – Group and treatment administered Table 2- Procedure for triglyceride determination Table 3- Procedure for HDL determination Table 4- Cholesterol CHOD-PAP assay Table 5- Showing the level of cholesterol, HDL, LDL and triglycerides in adult wistar rats.

1.0 Introduction 1.1 Medicinal Plants 1.2 Aim of Study CHAPTER TWO 2.0 Literature Review 2.1 Application of medicinal plants 2.1.1 Diocleareflexa Hook. F 2.2 Lipid Profile 2.2.1 Impacts of medicinal plants on lipid profile CHAPTER THREE 3.0 Materials and Methods 3.1 Apparatus and Equipments 3.2 Chemical Reagents 3.3 Collection and extraction of plant materials 3.3.1 Diocleareflexa Hook. F 3.4 Practical history and animal management 3.5 Drug administration 3.6 Sacrificing of rats 3.7 Biochemical analysis 3.7.1 Triglycerides 3.7.1.1 Principle 3.7.1.2 Reagent composition 3.7.1.3 Preparation of reagents 3.7.1.4 Procedure 3.7.2 HDL Cholesterol 3.7.2.1 Principle 3.7.2.2 Preparation of reagents 3.7.2.3 Procedure 3.8 Statistical analysis CHAPTER FOUR 4.0 Results CHAPTER FIVE 5.0 Discussion, Conclusion and Recommendation 5.1 Discussion 5.2 Conclusion 5.3 Recommendations References Appendix LIST OF FIGURES Figure1 – Diocleareflexa Hook. F Figure2 – Bar chart showing the level of cholesterol in adult wistar rats Figure3 – Bar chart showing the level of HDL in adult wistar rats Figure4 – Bar chart showing the level of LDL in adult wistar rats Figure5 – Bar chart showing the level of triglycerides in adult wistar rats LIST OF TABLE Table 1 – Group and treatment administered Table 2- Procedure for triglyceride determination Table 3- Procedure for HDL determination Table 4- Cholesterol CHOD-PAP assay Table 5- Showing the level of cholesterol, HDL, LDL and triglycerides in adult wistar rats.

 

Table Of Contents

<p> </p><div><p><em>Contents</em></p><p><em>Title page &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</em></p><p><em>Certification &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</em></p><p><em>Dedication &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; </em></p><p><em>Acknowledgements &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</em></p><p><em>Table of contents &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</em></p><p><em>List of Figures &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</em></p><p><em>List of Table &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; </em></p><p><em>Abstract &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</em></p><p>

Chapter ONE

</p><p>1.0 &nbsp; &nbsp; Introduction</p><p>1.1 &nbsp; &nbsp; Medicinal Plants</p><p>1.2 &nbsp; &nbsp; Aim of Study</p><p>

Chapter TWO

</p><p>2.0 &nbsp; &nbsp; Literature review</p><p>2.1 &nbsp; &nbsp; Medicinal Plants and their uses</p><p>2.1.1 <em>Mezoneuron benthamianum baill &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</em></p><p>2.2 &nbsp; &nbsp; Lipid Profile</p><p>2.2.1 Effect of medicinal plants on Lipid profile</p><p>

Chapter THREE

</p><p>3.0 &nbsp; &nbsp; Materials and Methods</p><p>3.1 &nbsp; &nbsp; Apparatus and Equipments</p><p>3.2 &nbsp; &nbsp; Chemical Reagents</p><p>3.3 &nbsp; &nbsp; Collection and extraction of plant materials</p><p>3.3.1 Mezoneuron benthamianum baill</p><p>3.4 &nbsp; &nbsp; &nbsp; Practical history and animal management</p><p>3.5 &nbsp; &nbsp; &nbsp; Drug administration</p><p>3.6 &nbsp; &nbsp; &nbsp; Sacrificing of Rats</p><p>3.7 &nbsp; &nbsp; &nbsp; Biochemical Analysis</p><p>3.7.1 &nbsp; &nbsp; Triglycerides</p><p>3.7.1.1 Principle</p><p>3.7.1.2 Reagent composition</p><p>3.7.1.3 Preparation of reagents</p><p>3.7.1.4 Procedure</p><p>3.7.2 HDL- Cholesterol</p><p>3.7.2.1 Principle</p><p>3.7.2.2 Preparation of reagents</p><p>3.7.2.3 Procedure</p><p>3.8 &nbsp; &nbsp; Statistical Analysis</p><p>

Chapter FOUR

</p><p>4.0 &nbsp; &nbsp; Results</p><p>

Chapter FIVE

</p><p>5.0 &nbsp; &nbsp; Discussion, Conclusion and Recommendation</p><p>5.1 &nbsp; &nbsp; Discussion</p><p>5.2 &nbsp; &nbsp; Conclusion</p><p>5.3 &nbsp; &nbsp; Recommendation</p><p>References</p><p>LIST OF FIGURES</p><p>Figure 1 –<em>Mezoneuron benthamianum baill &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</em></p><p>Figure 2 – Bar chart showing the level of cholesterol in adult wistar rats</p><p>Figure 3 – Bar chart showing the level of HDL in adult wistar rats</p><p>Figure 4 – Bar chart showing the level of LDL in adult wistar rats</p><p>Figure 5 – Bar chart showing the level of triglycerides in adult wistar rats</p><p>LIST OF TABLE</p><p>Table 1 – Groups and treatment administered</p><p>Table 2- Procedure triglyceride determination</p><p>Table 3 – Procedure for HDL determination</p><p>Table 4- Cholesterol CHOD-PAP assay</p><p>Table 5- Showing the level of cholesterol, HDL, LDL and</p><p>triglycerides in adult wistar rats.</p><p></p></div><h3></h3><br> <br><p></p>

Project Abstract

This research work was carried out to determine the effect of Menzoneuron benthamainum on fasting blood sugar level of adult wistar rats. Twenty wistar rats (both male and female) weighing between 140g to 160g were used for this research project. They were acclimatized for 14 days after which the animals were grouped into four groups of five rats each. Each animal were administered based on their body weight. The dose given to animals in group A was 300mg/kg body weight of Menzoneuron benthamainum, Group B was given 500mg/kg body weight of Menzoneuron benthamainum, Group C was given 700mg/kg body weight of Menzoneuron benthamainum and group D (control group) was given distilled water. The administration lasted for thirteen days. Blood samples were collected from the tail of the animals and the Lipid profile level for each of the animals was tested.

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