<p>
</p><div><p><strong>
Chapter ONE
</strong></p><p>1.0 Introduction and Literature review</p><p>1.1 Introduction</p><p>1.2 Literature review</p><p>1.2.1 History of arginase</p><p>1.3 Occurrence and distribution of arginase</p><p>1.3.1 Bacteria</p><p>1.3.2 Fungi</p><p>1.3.3 Lichens</p><p>1.3.4 Yeast</p><p>1.3.5 Insects</p><p>1.4 The substrate: Arginase</p><p>1.5 Scientific classification of local goat</p><p>1.6 Aim and Objectives</p><p>
Chapter TWO
</p><p>2.0 Materials and methods</p><p>2.1 Materials</p><p>2.1.1 Apparatus used</p><p>2.2 Methods</p><p>2.2.1 Assay</p><p>2.3.2 Protein determination</p><p>2.3.3 Purification procedure</p><p>
Chapter THREE
</p><p>3.0 Results</p><p>3.1 Purification and Homogeneity</p><p>3.2 Effect of metal ion on arginase from kidney of a local goat</p><p>
Chapter FOUR
</p><p>5.0 Discussion, Conclusion and Recommendation</p><p>5.1 Discussion</p><p>5.2 Conclusion</p><p>5.3 Recommendations</p><p>References.</p><p></p></div><h3></h3><br>
<br><p></p>
Project Abstract
This research is aimed at isolating, partially purifying and determination of arginase from the kidney of a local goat. Methods used in the isolation and partial purification of the enzyme include; Ammonium sulphate precipitation, dialysis.etc. Result from this work showed that arginase isolated from a local goat has a specific activity of 0.358 with a yield of 12.12% incubation of the enzyme with different metal ions shows that the enzyme is inhibited by Nacl and Bacl2.