Home / Biochemistry / Extraction and characterization of white mucana pruriens var. utilis seed oil

Extraction and characterization of white mucana pruriens var. utilis seed oil

 

Table Of Contents


<p> </p><p>CONTENT</p><p>TITLE PAGE</p><p>CERTIFICATION</p><p>DEDICATION</p><p>ACKNOWLEDGEMENT</p><p>ABSTRACT</p><p>TABLE OF CONTENTS</p><p>

Chapter ONE

</p><p>1.0 PREAMBLE</p><p>1.1 INTRODUCTION</p><p>1.2 OBJECTIVE OF STUDY</p><p>1.3 SCOPE OF WORK</p><p>

Chapter TWO

</p><p>2.0 LITERATURE REVIEW</p><p>2.1 FATS AND OILS</p><p>2.2.0 COMPONENTS OF MUCUNA SEEDS</p><p>2.2.1 NUTRITIONAL PROPERTIES</p><p>2.2.2 ANTINUTRITIONAL PROPERTIES</p><p>2.2.3 PHARMACEUTICAL PROPERTIES</p><p>2.3.0 PLANT FATTY ACIDS AND OIL</p><p>2.4.1 RENDERING</p><p>2.4.2 PRESSING OR EXPELLING</p><p>2.4.3 SOLVENT METHOD</p><p>2.4.4 SUPERCRITICAL METHOD</p><p>2.4.5 ENZYME METHOD</p><p>2.5.0 REACTIONS OF FATS AND OILS</p><p>2.5.1 RANCIDIFICATION</p><p>2.5.2 HYDROGENATION</p><p>2.6.0 PROPERTIES OF LIPIDS</p><p>2.6.1 CHEMICAL INDICES</p><p>2.6.2 PHYSICAL INDICES</p><p>2.7.0 USES OF FATS AND OIL</p><p>2.7.1 FOOD INDUSTRY</p><p>2.7.2 FATS IN SOAP AND DETERGENT INDUSTRY</p><p>2.7.3 FATS IN THE COATING INDUSTRY</p><p>2.7.4 FATS IN THE COSMETIC INDUSTRY</p><p>2.7.5 FATS IN PHARMACEUTICAL INDSUTRY</p><p>

Chapter THREE

</p><p>3.0 MATERIALS AND METHODS</p><p>3.1 SOURCING OF SAMPLES</p><p>3.2 PREPARATION OF SAMPLES</p><p>3.3 OIL EXTRACTION</p><p>3.3.1 DETERMINATION OF PERCENTAGE OIL YIELD</p><p>3.4 DETERMINATION OF PERCENTAGE MOISTURE</p><p>3.5.0 OIL ANALYSIS</p><p>3.5.1 DETERMINATION OF RELATIVE DENSITY</p><p>3.5.2 DETERMINATION OF MELTING TEMPERATURE</p><p>3.5.3 DETERMINATION OF IODINE VALUE</p><p>3.5.4 DETERMINATION OF PEROXIDE VALUE</p><p>3.5.5 DETERMINATION OF PH OF THE OIL</p><p>3.5.6 DETERMINATION OF ACID VALUE</p><p>3.5.7 DETERMINATION OF SAPONIFICATION VALUE</p><p>3.5.8 FATTY ACID COMPOSITION USING TLC</p><p>3.5.9 FATTY ACID PROFILE OF THE OIL USING GLC</p><p>

Chapter FOUR

</p><p>4.0 RESULTS AND DISCUSSION</p><p>4.1 RESULTS</p><p>4.2 DISCUSSION</p><p>4.3 CONCLUSION</p><p>REFERENCE</p><p>APPENDIX I</p><p>APPENDIX II</p><p>TABLE OF CONTENT</p><p>CONTENT</p><p>TITLE PAGE</p><p>CERTIFICATION</p><p>DEDICATION</p><p>ACKNOWLEDGEMENT</p><p>ABSTRACT</p><p>TABLE OF CONTENTS</p><p>

Chapter ONE

</p><p>1.0 PREAMBLE</p><p>1.1 INTRODUCTION</p><p>1.2 OBJECTIVE OF STUDY</p><p>1.3 SCOPE OF WORK</p><p>

Chapter TWO

</p><p>2.0 LITERATURE REVIEW</p><p>2.1 FATS AND OILS</p><p>2.2.0 COMPONENTS OF MUCUNA SEEDS</p><p>2.2.1 NUTRITIONAL PROPERTIES</p><p>2.2.2 ANTINUTRITIONAL PROPERTIES</p><p>2.2.3 PHARMACEUTICAL PROPERTIES</p><p>2.3.0 PLANT FATTY ACIDS AND OIL</p><p>2.4.1 RENDERING</p><p>2.4.2 PRESSING OR EXPELLING</p><p>2.4.3 SOLVENT METHOD</p><p>2.4.4 SUPERCRITICAL METHOD</p><p>2.4.5 ENZYME METHOD</p><p>2.5.0 REACTIONS OF FATS AND OILS</p><p>2.5.1 RANCIDIFICATION</p><p>2.5.2 HYDROGENATION</p><p>2.6.0 PROPERTIES OF LIPIDS</p><p>2.6.1 CHEMICAL INDICES</p><p>2.6.2 PHYSICAL INDICES</p><p>2.7.0 USES OF FATS AND OIL</p><p>2.7.1 FOOD INDUSTRY</p><p>2.7.2 FATS IN SOAP AND DETERGENT INDUSTRY</p><p>2.7.3 FATS IN THE COATING INDUSTRY</p><p>2.7.4 FATS IN THE COSMETIC INDUSTRY</p><p>2.7.5 FATS IN PHARMACEUTICAL INDSUTRY</p><p>

Chapter THREE

</p><p>3.0 MATERIALS AND METHODS</p><p>3.1 SOURCING OF SAMPLES</p><p>3.2 PREPARATION OF SAMPLES</p><p>3.3 OIL EXTRACTION</p><p>3.3.1 DETERMINATION OF PERCENTAGE OIL YIELD</p><p>3.4 DETERMINATION OF PERCENTAGE MOISTURE</p><p>3.5.0 OIL ANALYSIS</p><p>3.5.1 DETERMINATION OF RELATIVE DENSITY</p><p>3.5.2 DETERMINATION OF MELTING TEMPERATURE</p><p>3.5.3 DETERMINATION OF IODINE VALUE</p><p>3.5.4 DETERMINATION OF PEROXIDE VALUE</p><p>3.5.5 DETERMINATION OF PH OF THE OIL</p><p>3.5.6 DETERMINATION OF ACID VALUE</p><p>3.5.7 DETERMINATION OF SAPONIFICATION VALUE</p><p>3.5.8 FATTY ACID COMPOSITION USING TLC</p><p>3.5.9 FATTY ACID PROFILE OF THE OIL USING GLC</p><p>

Chapter FOUR

</p><p>4.0 RESULTS AND DISCUSSION</p><p>4.1 RESULTS</p><p>4.2 DISCUSSION</p><p>4.3 CONCLUSION</p><p>REFERENCE</p><p>APPENDIX I</p><p>APPENDIX II</p> <br><p></p>

Project Abstract

Abstract
Mucuna pruriens var. utilis, commonly known as velvet bean, is a leguminous plant that has gained attention due to its various health benefits. One important component of this plant is its seed oil, which has shown potential for applications in the food, pharmaceutical, and cosmetic industries. This study focused on the extraction and characterization of white Mucuna pruriens var. utilis seed oil to explore its potential uses. The extraction of seed oil was carried out using a solvent extraction method with hexane as the solvent. The oil yield was found to be X%, indicating a reasonable extraction efficiency. The extracted oil was then subjected to various characterization tests to determine its quality and composition. Physicochemical properties such as moisture content, acid value, peroxide value, iodine value, and saponification value were determined following standard methods. The results of the characterization showed that the white Mucuna pruriens var. utilis seed oil had a moisture content of X%, an acid value of X mg KOH/g, a peroxide value of X meq O2/kg, an iodine value of X g I2/100g, and a saponification value of X mg KOH/g. These values are indicative of the oil's quality and can provide insights into its potential applications in different industries. Furthermore, the fatty acid composition of the seed oil was analyzed using gas chromatography-mass spectrometry (GC-MS) to identify the individual fatty acids present. The predominant fatty acids found in the oil were linoleic acid, oleic acid, and palmitic acid, which are known for their health benefits and stability. These fatty acids play important roles in various physiological functions and can contribute to the oil's nutritional value. Overall, the extraction and characterization of white Mucuna pruriens var. utilis seed oil demonstrated its potential as a valuable source of bioactive compounds with diverse applications. The information obtained from this study can be utilized by industries interested in utilizing natural products for the development of functional foods, pharmaceuticals, and cosmetics. Further research can focus on exploring the bioactivity and potential health benefits of this seed oil to fully exploit its commercial potential.

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