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Isolation and identification of microoragnisms in cassava mill effluent in the soil

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of the Study
1.3 Problem Statement
1.4 Objective of the Study
1.5 Limitation of the Study
1.6 Scope of the Study
1.7 Significance of the Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Microorganisms
2.2 Importance of Microorganisms in the Environment
2.3 Microorganisms in Cassava Mill Effluent
2.4 Effects of Cassava Mill Effluent on Soil Microorganisms
2.5 Methods for Isolation and Identification of Microorganisms
2.6 Previous Studies on Microorganisms in Soil
2.7 Impact of Microorganisms on Soil Health
2.8 Role of Microorganisms in Nutrient Cycling
2.9 Microbial Diversity in Soil
2.10 Future Research Directions in Microbial Ecology

Chapter THREE

3.1 Research Methodology Overview
3.2 Sampling Techniques
3.3 Isolation of Microorganisms
3.4 Identification of Microorganisms
3.5 Microbiological Analysis Methods
3.6 Data Collection Procedures
3.7 Statistical Analysis Techniques
3.8 Quality Control Measures

Chapter FOUR

4.1 Overview of Research Findings
4.2 Distribution of Microorganisms in Cassava Mill Effluent
4.3 Diversity of Microorganisms in Soil Samples
4.4 Comparison of Microbial Communities in Different Samples
4.5 Factors Influencing Microbial Composition
4.6 Interaction Between Microorganisms and Soil Properties
4.7 Implications of Findings on Soil Health
4.8 Recommendations for Future Research

Chapter FIVE

5.1 Conclusion and Summary
5.2 Summary of Research Findings
5.3 Contributions to the Field
5.4 Implications for Environmental Management
5.5 Limitations of the Study
5.6 Recommendations for Further Research
5.7 Conclusion and Closing Remarks

Project Abstract

The Isolation and identification of microorganisms in cassava mill effluent in the soil in Orji, Imo State, Nigeria were carried out, soil samples were collected from site polluted with cassava mill effluent and also from adjacent site not polluted with cassava effluent to serve as control. The overall population of microorganisms in cassava mill effluent was determined. Isolation and characterization of succession of microorganism in cassava mill effluent was studied microbial count, Bacterial counts and fungal counts were also determined using a pour plate method. The microbial count ranges from 2.2 X 105 – 2.0 X 105 for THBC, 1.1 X 105 – 1.2 X 105 for TCC and the TFC ranges from 1.2 X 105-1.5 X 105. The bacteria isolated were Klebsiella spp., Escherichia coli , Pseudomonas spp., Staphylococcus aureus, and Streptococcus spp. while the fugal isolates include Penicillium spp., Aspergillus spp., mucor spp., and yeast cells Candida spp. the cassava mill effluent should be treated before discharge into the environment to prevent pollution.



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