Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Protein and Iron Metabolism
2.2 Malaria Parasite (Plasmodium berghei, berghei)
2.3 Tetracycline: Mechanism of Action and Side Effects
2.4 Relationship Between Malaria Infection and Protein/Iron Levels
2.5 Previous Studies on Protein and Iron Levels in Malaria-Infected Mice
2.6 Impact of Tetracycline Treatment on Protein and Iron Levels
2.7 Factors Affecting Serum Protein and Iron Levels
2.8 Importance of Monitoring Protein and Iron in Disease Conditions
2.9 Techniques for Estimating Total Serum Protein and Iron Levels
2.10 Role of Protein and Iron in Immune Response

Chapter THREE

3.1 Research Design and Rationale
3.2 Selection of Animal Model
3.3 Sample Collection and Preparation
3.4 Experimental Protocol for Malaria Infection
3.5 Administration of Tetracycline Treatment
3.6 Measurement of Total Serum Protein Levels
3.7 Estimation of Serum Iron Levels
3.8 Data Analysis Techniques

Chapter FOUR

4.1 Overview of Study Findings
4.2 Effects of Malaria Infection on Serum Protein and Iron Levels
4.3 Impact of Tetracycline Treatment on Protein and Iron Metabolism
4.4 Comparison of Protein and Iron Levels in Infected and Treated Mice
4.5 Correlation Between Parasite Load and Protein/Iron Levels
4.6 Variability in Protein and Iron Responses among Mice
4.7 Discussion on Factors Influencing Protein and Iron Levels
4.8 Implications of Findings for Malaria Treatment Strategies

Chapter FIVE

5.1 Summary of Research Findings
5.2 Conclusion and Interpretation of Results
5.3 Contributions to Existing Knowledge
5.4 Recommendations for Future Research
5.5 Practical Applications and Implications
5.6 Closing Remarks