Home / Biochemistry / Effective use of a tropical hop named bitter leaf [vernonia amygdalina] extract as a means of extending the shelf – life of locally brewed millet beer

Effective use of a tropical hop named bitter leaf [vernonia amygdalina] extract as a means of extending the shelf – life of locally brewed millet beer

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Literature Review
2.2 Historical Perspective
2.3 Conceptual Framework
2.4 Theoretical Framework
2.5 Previous Studies on the Topic
2.6 Current Trends and Gaps in Research
2.7 Comparative Analysis
2.8 Key Findings in Literature
2.9 Implications of Literature Review
2.10 Summary of Literature Reviewed

Chapter THREE

3.1 Research Methodology Overview
3.2 Research Design and Approach
3.3 Sampling Techniques
3.4 Data Collection Methods
3.5 Data Analysis Procedures
3.6 Research Ethics Considerations
3.7 Reliability and Validity
3.8 Limitations of Methodology

Chapter FOUR

4.1 Overview of Findings
4.2 Demographic Profile of Participants
4.3 Data Analysis Results
4.4 Patterns and Themes Identified
4.5 Interpretation of Findings
4.6 Comparison with Research Objectives
4.7 Discussion on Key Findings
4.8 Recommendations for Future Research

Chapter FIVE

5.1 Conclusion and Summary
5.2 Recap of Research Objectives
5.3 Key Findings Recap
5.4 Implications of Findings
5.5 Contributions to Knowledge
5.6 Recommendations for Practice
5.7 Suggestions for Further Research
5.8 Closing Remarks

Project Abstract

Abstract
The research project focuses on exploring the potential use of bitter leaf (Vernonia amygdalina) extract as a natural preservative to extend the shelf-life of locally brewed millet beer. Millet beer is a traditional alcoholic beverage consumed widely in various African communities, but its short shelf-life limits its market reach and commercial viability. Bitter leaf is a tropical herb known for its antimicrobial properties due to the presence of bioactive compounds like alkaloids, saponins, and flavonoids. This study aims to investigate the effectiveness of bitter leaf extract in inhibiting the growth of spoilage microorganisms in millet beer, thereby prolonging its shelf-life. The research methodology involves the extraction of bitter leaf bioactive compounds using suitable solvents like ethanol or water. The antimicrobial activity of the extract will be evaluated through in vitro tests against common beer spoilage microorganisms such as Saccharomyces cerevisiae and Lactobacillus spp. The most effective extraction method and concentration of bitter leaf extract will be determined through experimentation to achieve optimal preservation of millet beer. Furthermore, sensory evaluation tests will be conducted to assess the impact of bitter leaf extract on the flavor, aroma, and overall quality of the millet beer. Consumer acceptance of the preserved beer will also be analyzed to ensure that the addition of bitter leaf extract does not negatively affect its organoleptic properties. Additionally, the nutritional content of the preserved beer will be analyzed to ensure that the bioactive compounds from bitter leaf do not alter its composition significantly. The potential benefits of using bitter leaf extract as a natural preservative for millet beer include extending its shelf-life, reducing spoilage, and enhancing the microbial safety of the product without the need for synthetic additives. If successful, this research could contribute to the development of a cost-effective and sustainable method to improve the marketability of locally brewed millet beer, benefiting both consumers and producers in the beverage industry. Overall, the project aims to explore the practical application of bitter leaf extract in food preservation and promote the utilization of natural ingredients to enhance food safety and quality.

Project Overview

1.0   INTRODUCTION

Beer, alcoholic brewery made from a Cerael grains known as barley and Millet.A process called Fermentation in which Microscopic fungi known as yeast is used to consume sugers in the grains to make Beers,converting them to alcohol and carbondioxide gas.This chemical process typically produce beers with an adequate level of alcohol content of about 2-6%.Over an aggregate of 70 types of beer are available in the modern worlds todays.Each style of beer derives its unique characteristics and differences in its brewing process.Four basic ingredients are used in brewing,which includes;grains, hops,[bitterleaves as a hop’s substitute] Yeast and water,Grains like Millet contain the natural sugers required for fermentation.It also provides beer with flavour,colour,body and texture.Hops are small,green,cone-shaped flowers from the hop plant,a vine related to the nettle plant over 50 varieties of hops are grown throughout the world, mostly in Europe,Australia and North America.But the hops we want to used is bitterleave acting as hop’s substitute.It is mostly grown here in Africa.Hops provides the Beer with spicy, bitter flavour and contribute natural substances that prevent bacteria from spoiling beer. Two species of Yeast ;Saccharomyces cerevisiae and Saccharomyces uvarum and baker’s yeast.Each yeast species is used in a slightly different method of fermentation and produces a distinct type of beer. Throughout history, where ever Cereal grains were grown, Humans made beer with beverage for them. They used Barley in Egypt, Millet and Sorghum in other parts of Africa, beer brewing is a major industrial revolution world wide. In 2002,In the United States,1800brewers,produced almost 175 millions barrels of beer.Beer is deeply interlined in the fabric of Society, from the Economic to the intangible locally owned breweries deliver social values better than global companies. Since early times beer which is the products of brewing processes has played an important role in our society, It bring people together in local pubs and breweries generating Camaraderie within Communities. As technology has progress brewing techniques have been developed and refined. As a result, we are currently consuming the finest beers ever produced. This is due to our increased knowledge in the fields of Biotechnology, Microbiology, and Chemistry.TROPICAL HOP

1.1AIMOFSTUDY / PROJECT

The aim of study is primarily to have a capture of the effect use of a tropical hop named bitter leaf(Vernonia Amygdalina) extract as a means of extending the shelf–life of a locally millet beer.In the course of achieving this goal, the ingredients used in brewing industries are taken into consideration together with various analysis carried on beer samples. Finally, beer should be of an adequate composition and texture in order to satisfy consumers need and sensitivity on the disadvantages of beer abuse to the health and body.



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