Home / Biochemistry / THE OXIDATIVE STRESS STATUS OF RATS FED ON OIL BEAN SEED MEAL

THE OXIDATIVE STRESS STATUS OF RATS FED ON OIL BEAN SEED MEAL

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Oxidative Stress
2.2 Oil Bean Seed Meal as Animal Feed
2.3 Effects of Oil Bean Seed Meal on Rat Health
2.4 Antioxidants and Their Role in Oxidative Stress
2.5 Previous Studies on Oxidative Stress in Animal Models
2.6 Impact of Diet on Oxidative Stress
2.7 Role of Nutritional Supplements in Reducing Oxidative Stress
2.8 Methods to Measure Oxidative Stress Levels
2.9 Role of Lipids in Oxidative Stress
2.10 Impact of Oxidative Stress on Cellular Function

Chapter THREE

3.1 Research Design
3.2 Sampling Techniques
3.3 Data Collection Methods
3.4 Data Analysis Procedures
3.5 Ethical Considerations
3.6 Instrumentation Used
3.7 Variables Considered
3.8 Research Framework

Chapter FOUR

4.1 Overview of Research Findings
4.2 Analysis of Oxidative Stress Levels in Rats
4.3 Comparison of Control and Experimental Groups
4.4 Impact of Oil Bean Seed Meal on Antioxidant Levels
4.5 Effects of Duration of Feeding on Oxidative Stress
4.6 Correlation between Diet and Oxidative Stress Biomarkers
4.7 Discussion on Lipid Peroxidation Levels
4.8 Interpretation of Cellular Damage from Oxidative Stress

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusion
5.3 Implications of the Study
5.4 Recommendations for Future Research
5.5 Contribution to the Field

Project Abstract

This study was on oxidative stress status of rat fed with Pentraclethra macrophylla, otherwise known as African oil bean seed oil in English or Ugba in Igbo. Sixteen male rats were distributed into four groups. 1 (control), then group 2, 3, and 4 as test groups. They were fed with their formulated meal (5%, 10%, 20% inclussions) for 28 days. Group 1 was the control and were fed with the normal feed, while group 2, 3 and 4 which were the test groups were fed with test feed formula.. The parameters determined were MDA concentrations and catalase activity. Serum MDA significantly increased (p<0.05) while the catalase activity significantly decreased (p<0.05). This finding may be clinically significant to individuals with predisposition to increased generation of reactive oxygen species (ROS) and other degenerative diseases.


Project Overview


1.0. INTRODUCTION
Ugba also called ukpaka is a popular food delicacy in Nigeria especially among Igbo ethnic group. It is rich in protein and is obtained by a solid state fermentation of the seed of African oil bean tree (Pentaclethra macrophylla Benth).
The natural fermentation of the seed which at present is still done at the house-hold level, renders the production nutritious, palatable and non-toxic (Enujiugha, 2002).
Its production, like many African fermented foods depends, entirely on mixed fermentation by microorganism from diverse source.
Pentaclethra macrophylla Benth is a large woody plant abundant in the rain forest areas of west and central Africa. It’s origin in Nigeria is believed to be around 1937 (Ladipo, 1984); where it is found in the South Nigeria, (Mbajunwa et al., 1998).
―Ugba‖ Pentaclethra macrophylla Benth belongs to the Family Leguminosae and sub-family Microsoideae (Keay, 1989 and NFTA, 1995).
Ugba seeds are irregular and oval; they are flat, black and hard pods. It is composed of oil, protein and small amounts of carbohydrate (Obeta, 1982

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