Project Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play crucial roles in the post-transcriptional regulation of gene expression. In recent years, there has been growing interest in understanding how miRNAs are involved in cancer development and progression. This research project aims to investigate the role of miRNAs in regulating gene expression in cancer cells, with a focus on how dysregulation of miRNAs may contribute to tumorigenesis. The study will begin with a comprehensive literature review to provide a background on the current understanding of miRNA functions in cancer. This will include an exploration of the mechanisms by which miRNAs regulate gene expression, as well as the specific miRNAs that have been implicated in various types of cancer. By synthesizing existing knowledge, the review will highlight the gaps in understanding that this research seeks to address. The research methodology will involve the use of cancer cell lines to investigate the expression levels of specific miRNAs and their target genes. Various experimental techniques, such as quantitative real-time polymerase chain reaction (qPCR) and luciferase reporter assays, will be employed to assess the regulatory effects of miRNAs on gene expression. Additionally, bioinformatics tools will be utilized to predict miRNA-target interactions and identify potential pathways affected by dysregulated miRNAs. The findings from this study will be discussed in detail in Chapter 4, focusing on the specific miRNAs that show altered expression patterns in cancer cells and their putative target genes. The potential implications of these findings for cancer biology and therapeutics will be considered, with a view towards identifying novel biomarkers or therapeutic targets for further exploration. In conclusion, this research project aims to provide valuable insights into the role of miRNAs in regulating gene expression in cancer cells. By elucidating the molecular mechanisms underlying miRNA-mediated gene regulation, this study seeks to contribute to the understanding of cancer pathogenesis and potentially identify new avenues for targeted cancer therapy.

Project Overview