Project Abstract

This project emphasizes the critical need to explore the potential of medicinal plants as a source of novel antimicrobial compounds. The rapid emergence of multidrug-resistant pathogens poses a significant threat to global public health, necessitating the development of alternative therapeutic strategies. Medicinal plants have long been recognized for their rich repertoire of bioactive secondary metabolites, many of which exhibit potent antimicrobial properties. By characterizing these antimicrobial compounds, this project aims to contribute to the discovery of new antimicrobial agents that can be leveraged to combat the growing challenge of antimicrobial resistance. The project will focus on the systematic screening and isolation of antimicrobial compounds from selected medicinal plants, with a particular emphasis on traditional herbal remedies used in various cultures for the treatment of infectious diseases. A comprehensive literature review will be conducted to identify promising plant species with reported antimicrobial activities, and these will be subjected to detailed phytochemical and biological investigations. The first phase of the project will involve the extraction, fractionation, and purification of plant extracts using various chromatographic techniques, such as column chromatography, thin-layer chromatography, and high-performance liquid chromatography. The antimicrobial potency of the isolated fractions will be evaluated against a panel of clinically relevant bacterial and fungal pathogens, including both drug-sensitive and drug-resistant strains. This screening process will help identify the most promising antimicrobial compounds for further characterization. The second phase of the project will focus on the structural elucidation and characterization of the active antimicrobial compounds. Advanced analytical techniques, such as nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and infrared (IR) spectroscopy, will be employed to determine the chemical structures of the isolated compounds. The mode of action, target specificity, and underlying mechanisms of the antimicrobial activities will also be investigated through a combination of biochemical, microbiological, and molecular biology techniques. The project will also explore the potential synergistic effects of combining the identified antimicrobial compounds with conventional antibiotics. This approach could lead to the development of novel combination therapies that can enhance the efficacy of existing antimicrobial agents and potentially overcome the challenge of antimicrobial resistance. Throughout the project, special attention will be paid to the sustainable and ethical sourcing of plant materials, as well as the development of environmentally friendly extraction and purification protocols. The ultimate goal is to generate a comprehensive database of antimicrobial compounds from medicinal plants, which can serve as a valuable resource for the pharmaceutical industry, academia, and the broader scientific community. In conclusion, this project represents a multidisciplinary effort to harness the untapped potential of medicinal plants in the search for new antimicrobial agents. By characterizing the antimicrobial compounds from these natural sources, the project aims to contribute to the development of innovative and effective strategies for combating the global crisis of antimicrobial resistance.

Project Overview